stat3 h 190 polyclonal antibody Search Results


mcm5 antibody  (NSJ Bioreagents)
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NSJ Bioreagents mcm5 antibody
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gapdh antibody (1d4)  (Bio-Techne corporation)
97
Bio-Techne corporation gapdh antibody (1d4)
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α-stat3  (Santa Cruz Biotechnology)
90
Santa Cruz Biotechnology α-stat3
α Stat3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stat3 h 190  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology stat3 h 190
Stat3 H 190, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti stat3 h 190  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti stat3 h 190
Anti Stat3 H 190, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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stat3  (Cell Signaling Technology Inc)
91
Cell Signaling Technology Inc stat3
AC-73 inhibits activation of the CD147/ERK1/2 and <t>STAT3</t> signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.
Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti stat3 h 190  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology rabbit polyclonal anti stat3 h 190
AC-73 inhibits activation of the CD147/ERK1/2 and <t>STAT3</t> signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.
Rabbit Polyclonal Anti Stat3 H 190, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-phospho-c-myc  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-phospho-c-myc
AC-73 inhibits activation of the CD147/ERK1/2 and <t>STAT3</t> signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.
Anti Phospho C Myc, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-phospho-stat3 clone 9e12  (Millipore)
90
Millipore anti-phospho-stat3 clone 9e12
AC-73 inhibits activation of the CD147/ERK1/2 and <t>STAT3</t> signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.
Anti Phospho Stat3 Clone 9e12, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti-total erk1/2  (Millipore)
90
Millipore anti-total erk1/2
AC-73 inhibits activation of the CD147/ERK1/2 and <t>STAT3</t> signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.
Anti Total Erk1/2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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flag (m2  (Millipore)
90
Millipore flag (m2
AC-73 inhibits activation of the CD147/ERK1/2 and <t>STAT3</t> signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.
Flag (M2, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


AC-73 inhibits activation of the CD147/ERK1/2 and STAT3 signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.

Journal: Journal of Crohn's & Colitis

Article Title: CD147 Targeting by AC-73 Induces Autophagy and Reduces Intestinal Fibrosis Associated with TNBS Chronic Colitis

doi: 10.1093/ecco-jcc/jjac084

Figure Lengend Snippet: AC-73 inhibits activation of the CD147/ERK1/2 and STAT3 signalling pathways and induces autophagy in mice with chronic TNBS colitis. [A, B] Western blot analysis of [A] ERK1/2 total [pan ERK1/2] and phosphorylated ERK1/2 [pERK1/2] protein expression levels, [B] STAT3 total [pan STAT3] and phosphorylated STAT3 [pSTAT3] protein expression levels. [A, B] Left: densitometric data are presented as the ratio of activated ERK to total ERK [pERK1/2/pan ERK1/2] and the ratio of activated STAT3 to total STAT3 [p-STAT3/pan STAT3]. [C] Western blot analysis of autophagy-related protein LC3 and its conversion from LC3-I to LC3-II in TNBS+AC-73, TNBS+V and ETOH+V mice; one representative western blot out of three is shown with densitometric data of the LC3B-II to LC3B-I ratio; GAPDH is an internal control of total protein extracts; right: densitometric data [A.U. = arbitrary units] represented as individual plots from at least: ten TNBS-treated mice per group and five ethanol-treated mice. Lines indicate median values. ns [not significant], * p <0.05, ** p <0.01 from a Mann–Whitney test.

Article Snippet: Antibodies used were: CD147 monoclonal antibody (EMMPRIN/CD147 [B5] sc-46700, Santa Cruz); TGF-β1 monoclonal antibody (TGFβ1[TB21] sc-52893, Santa Cruz); α-SMA polyclonal antibody [Cell Signaling Technology, Ma, USA]; for total STAT3 protein detection, STAT3 [H-190] polyclonal antibody [sc-7179, Santa Cruz]; phospho-STAT3-S727 and phospho-ERK1/2 [p44/p42 MAPK] polyclonal antibodies [Immunological Sciences]; for total ERK1/2 protein detection, p44/42 MAPK [Erk1/2] polyclonal antibody [9102, Cell Signaling Technology]; GAPDH polyclonal antibody [Sigma-Aldrich] was used as an internal control of the loaded amounts of total proteins.

Techniques: Activation Assay, Western Blot, Expressing, MANN-WHITNEY